FIGURE 4.

Rupintrivir and quercetin suppress RdRP synthesis in DWV-infected pupal head tissue. (A) Effect of rupintrivir (1 and 5 μM) and quercetin (1 and 5 mM) on RdRP synthesis in DWV-infected pupal head tissues. DMSO was used as control. (B) The band intensity of the RdRP precursor was compared between DMSO and rupintrivir or quercetin at different concentrations through one-tailed Welch’s t-test and P-values between DMSO and 5 μM rupintrivir or 5 mM quercetin are <0.003 and <0.002, respectively (**). Mean values ± SD (error bars) are shown (n = 6). (C) Luminescence generated by the luciferase activity dependent on the intracellular ATP level was compared between the dispersed pupal head cells treated with DMSO, rupintrivir, or quercetin at the indicated concentrations. Mean values ± SD (error bars) are shown (n = 6). **This represents statistical significance.