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. 2021 Jan 23;9:100096. doi: 10.1016/j.mtbio.2021.100096

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© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 4 — Study of osteogenic differentiation for hBM-MSCs encapsulated in PLMA hydrogels cultured with basal medium, osteogenic medium, and 1% MSNCaPDex PLMA hydrogels in basal medium. In vitro (A) ALP activity and (B) BMP-2 quantification. Representative fluorescence images after 21 d of: (C) osteopontin (green channel: Alexa Fluor 488, osteopontin staining; blue channel: DAPI, nucleus staining) and (D) hydroxyapatite (green channel: hydroxyapatite staining; blue channel: DAPI, nucleus staining). Scale bar: 100 μm. (E) Representative optical microscopy images of Alizarin Red S staining of calcium deposits after 21 d. Scale bar: 500 μm. Data represent mean ± s.d. (n = 3). ∗ = p<0.05, ∗∗ = p<0.01, ∗∗∗ = p<0.001, ∗∗∗∗ = p<0.0001. Symbols above bars are compared to those of basal medium.