Table 1.
Primers used for qPCR.
| Gene | Forward primer (5' → 3′) | Reverse primer (5' → 3′) |
|---|---|---|
| Acc | TCCTTGTGGATTACGGACTGCGAA | CCGGCTCCAAGTGGCGGTA |
| Acs | GAAGATCAAGCGAGGCTCCA | CCTTCCTGCATTCCATCGTC |
| Aif1 | ATCAACAAGCAATTCCTCGATGA | CAGCATTCGCTTCAAGGACATA |
| Agrp | GCTGCAGAAGGCAGAAGC | GACTCGTGCAGCCTTACACA |
| Bsx | TTTCTGACTCGCAGCTCTCC | CTGGAACGTTTTCACCT |
| Cb1 | TGCTTGCGATCATGGTGTAT | TGTCTCAGGTCCTTGCTCCT |
| Cpt1a | ATGTGGACCTGCATTCCTTC | GAACTTGCCCATGTCCTTGT |
| Cpt1c | AGAAGTAGACTCAGCTCG | CCAGAGATGCCTTTTCCAG |
| Fasn | CCCTTGATGAAGAGGGATCA | ACTCCACAGGTGGGAACAAG |
| Foxo | GCAACGCGTGGGGCAACCTGT | GGGCACGCTCTTCACCATCCACTC |
| Gfap | GTTTCATCTTGGAGCTTCTGC | GGAGGTGGAGAGGGACAAC |
| Ghsr | AAGATGCTTGCTGTGGTGGT | AAAGGACACCAGGTTGCAGT |
| Lipe | CCGTTCCTGCAGACTCTCTC | CCACGCAACTCTGGGTCTAT |
| Mgll | CAGAGAGGCCAACCTACTTTTC | ATGCGCCCCAAGGTCATATTT |
| Npy | TGGACTGACCCTCGCTCTAT | TGTCTCAGGGCTGGATCTCT |
| Npyy1r | CGGCGTTCAAGGACAAGTAT | CGTTGATTCGTTTGGTCTCA |
| Pnpla2 | CACAGCGCTGGTCACTGGGG | CCGGGCCTCCTTGGACACCT |
| Pomc | AGAGAGCTGCCTTTCCGCGAC | GCAGGAGGGCCAGCAACAGG |
| Sirt1 | CGCAGTCTCCAAGGAGCTCTACA | TGGATCTTCTCTGAAAGTGAGACCAGT |
| Tbp | TCAAACCCAGAATTGTTCTCC | GGTAGATGTTTTCAAATGCTTCA |
| Ucp2 | CCTACAAGACCATTGCACGA | TGTCATGAGGTTGGCTTTCA |
| Ywhaz | AGACGGAAGGTGCTGAGAAA | GAAGCATTGGGGATCAAGAA |