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. 2020 Dec 16;11(4):1139–1161. doi: 10.1016/j.jcmgh.2020.12.007

Figure 5.

Figure 5

Validation of landmark genes by immunolabeling and expression of new liver EC landmark genes in control mice. (A) Immunolabeling of zone-associated landmark genes in frozen liver sections from endothelial-GFP reporter mice. Red indicates landmark genes, green indicates GFP (ECs or IL7 for LyECs), blue indicates DAPI (nuclei). Scale bar: 40 μm. Images were taken using a Zeiss fluorescence microscope. (B) Violin plots showing expression levels of new zone-associated landmark genes in liver ECs 1-5. Each dot represents a single cell. White lines indicate median expression values. BD, bile duct; CV, central vein; HA, hepatic artery; PV, portal vein.