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. 2020 Dec 16;11(4):1139–1161. doi: 10.1016/j.jcmgh.2020.12.007

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© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Cluster 6 represents LyEC. (A) Feature plots showing relative distributions of established LyEC marker genes (Lyve1, Flt4, Pdpn, and Prox1) among all the liver ECs. Expression levels of these LyEC marker genes identified cluster 6 as LyECs. (B) Comparison of LyEC maker gene expression between zone 2 LSECs and LyECs. Zone 2 LSECs were chosen for the comparison because of the highest levels of Lyve1 and Flt4 that they expressed among 3 LSEC populations. Each dot represents a single cell. White lines indicate median expression values. (C) Feature plots showing some of the genes found only in cluster 6, which thus have the potential as new LyEC markers and could help to distinguish LyECs from LSECs. (D) qPCR analysis to validate unique LyEC markers (distinct from LSECs) identified in this scRNA-seq analysis. Human primary LSECs and LyECs were used for qPCR analysis. n = 3. ∗∗∗∗P < .0001. qPCR analysis was repeated 3 times to confirm this finding.