Fig. 14.

Comparison of real and virtual histology. A) Light microscopy of rat ventromedial WM in thoracic spinal cord. Reproduced from (Baxi et al., 2015), scale bar 2 ​μm. B) Two virtual histological slices from a ConFiG generated phantom. Phantoms are rendered to have similar colours to electron microscopy studies. The exact contrast and fibre bundle configurations are different between the real and virtual tissues, but the general morphology of the myelinated axons are captured well using ConFiG as highlighted by corresponding boxes. Yellow and Blue: axons severely deformed between other axons. Red: Pockets of empty space forming. Green: Largely circular axon surrounded by other axons deforming around it. Scale bar 2 ​μm.