Figure S1.

Uhrf1 did not affect the development and homeostasis of the immune system. (A) Genotyping PCR of myeloid cell-conditional Uhrf1 WT (Uhrf1^+/+lyz2^Cre/+), Uhrf1^MKO (Uhrf1^fl/fllyz2^Cre/+), and heterozygous (Uhrf1^fl/+lyz2^Cre/+) mice to amplify WT and floxed alleles and Cre-specific primers for the Lyz2^Cre DNA. (B and C) Flow cytometry analysis of macrophages (CD11b⁺F4/80⁺) and neutrophils (CD11b⁺GR-1⁺) in BM and spleen (Spl) from 6–8-wk-old WT and Uhrf1^MKO mice (n = 3). (D–G) Flow cytometry analysis of the absolute numbers of different immune cells in the spleen (D and E) and naive and memory T cells (F and G) from WT or USP16^MKO mice (n = 3). All FACS data are presented in a representative plot and summary graph of the subpopulation percentages. All data are representative of three independent experiments. The bars and error bars show the means ± SEMs. Significance was determined by a two-tailed Student’s t test. BMC, BM cells; CM, central memory; EM, effector memory; iLN, inguinal LN; M, total memory; N, naïve; SP, splenocytes.