Figure S2.

Uhrf1 deficiency in myeloid cells caused an autoimmune feature. (A) Uhrf1^ER-Cre MEFs were incubated with DMSO or 4-OH for 72 h and then infected with VSV-GFP at a MOI of 0.1 for 24 h Data are presented as a representative picture, showing the infected (GFP⁺) and total (bright-field) cells. Scale bar, 200 µm (n = 3). (B) The summary graph of flow cytometric quantification of the infected cells (n = 3). (C) Immunofluorescence microscopy of the deposition of IgM and IgG in glomeruli (arrows) of kidney sections from 8-mo-old WT and Uhrf1^MKO mice. Original magnification, ×10. Scale bar, 1,000 µm. (D) ELISA of the autoantibodies antinuclear antigen (ANA) and antibody to double-stranded DNA (dsDNA) in the serum of these aging WT and Uhrf1^MKO mice (n = 4). (E) Flow cytometric analysis of the percentages of IFN-γ– and IL-17–producing CD4⁺ T cells in the spleens and inguinal LNs (iLNs) of WT and Uhrf1^MKO mice (n = 4). All FACS data are presented in a representative plot and summary graph of the subpopulation percentages. All data are representative of three independent experiments. The bars and error bars show the means ± SEMs. Significance was determined by two-tailed Student’s t test. *, P < 0.05, **, P < 0.01; ***, P < 0.005.