Fig. 2.

Ag85A-specific antibody responses in the circulation and airways of immunised and control mice at 4, 8 and 20 weeks post vaccination. Mice were vaccinated as shown in Fig. 2a and the samples were collected at (b-c) 4 weeks, (d-e) 8 weeks and (f-g) 20 weeks after vaccination completed. (b, d and f) Serum and (c, e and g) BAL samples were analysed using ELISA specific to Ag85A. (b) Total IgG in serum samples at 1:1350 dilution and (c) IgA in undiluted BAL samples were measured at 4 weeks post vaccination. At 8 and 20 week-time points, (d and f) total IgG and (e and g) IgA were presented respectively. Each symbol represents one animal and the line is the median of each group (n = 4–6 mice). Data are representative of two independent experiments. Statistical significance was determined using Kruskal-Wallis test with Dunn's multiple comparisons test. *, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001.