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. Author manuscript; available in PMC: 2021 Feb 24.
Published in final edited form as: Cell Rep. 2021 Feb 2;34(5):108718. doi: 10.1016/j.celrep.2021.108718

Figure 1. Gnao1 expression in dMSNs required for motor learning in mice.

Figure 1.

(A) Schematic of targeting Gnao1 deletion in striatal neurons.

(B) Hindlimb clasping pathology score for Gnao1flox/flox (WT; n = 7) and Gnao1flox/flox:RGS9Cre (Str KO; n = 4) mice (nonparametric t test p = 0.0030, Kolmogorov-Smirnov D = 1.000).

(C) Latency to fall off a rotating beam while walking backward for Gnao1flox/flox (WT; n = 7) and Gnao1flox/flox:RGS9Cre (Str KO; n = 4) mice (nonparametric t test p = 0.0030, Kolmogorov-Smirnov D = 1.000).

(D) Ledge test pathology score for Gnao1flox/flox (WT; n = 7) and Gnao1flox/flox:RGS9Cre (Str KO; n = 4) mice (nonparametric t test p = 0.0030, Kolmogorov-Smirnov D = 1.000).

(E) Accelerating rotarod learning rate for Gnao1flox/flox (WT; n = 12) and Gnao1flox/flox:RGS9Cre (Str KO; n = 10) mice (nonparametric t test p = 0.0157, Kolmogorov-Smirnov D = 0.6667).

(F) Schematic of targeting Gnao1 deletion in dMSNs.

(G) Hindlimb clasping pathology score for Gnao1flox/flox (WT; n = 8) and Gnao1flox/flox:Drd1aCre (dMSN KO; n = 7) mice (p = 0.5692, Kolmogorov-Smirnov D = 0.2321).

(H) Latency to fall off a rotating beam while walking backward for Gnao1flox/flox (WT; n = 8) and Gnao1flox/flox:Drd1aCre (dMSN KO; n = 7) mice (nonparametric t test p = 0.6476, Kolmogorov-Smirnov D = 0.3214).

(I) Ledge test pathology score for Gnao1flox/flox (WT; n = 7) and Gnao1flox/flox:Drd1aCre (dMSN KO; n = 8) mice (nonparametric t test p = 0.2821, Kolmogorov-Smirnov D = 0.3036).

(J) Accelerating rotarod learning rate for Gnao1flox/flox (WT; n = 11) and Gnao1flox/flox:Drd1aCre (dMSN KO; n = 8) mice (nonparametric t test p = 0.0041, Kolmogorov-Smirnov D = 0.8182).

(K) Schematic of targeting Gnao1 deletion in iMSNs.

(L) Hindlimb clasping pathology score for Gnao1flox/flox (WT; n = 8) and Gnao1flox/flox:Drd2Cre (iMSN KO; n = 10) mice (p < 0.0001, Kolmogorov-Smirnov D = 1.000).

(M) Latency to fall off a rotating beam while walking backward for Gnao1flox/flox (WT; n = 8) and Gnao1flox/flox:Drd2Cre (iMSN KO; n = 10) mice (nonparametric t test p = 0.0012, Kolmogorov-Smirnov D = 0.8000).

(N) Ledge test pathology score for Gnao1flox/flox (WT; n = 8) and Gnao1flox/flox:Drd2Cre (iMSN KO; n = 10) mice (nonparametric t test p < 0.0001, Kolmogorov-Smirnov D = 1.000).

(O) Accelerating rotarod learning rate for Gnao1flox/flox (WT; n = 8) and Gnao1flox/flox:Drd2Cre (iMSN KO; n = 7) mice (nonparametric t test p = 0.4218, Kolmogorov-Smirnov D = 0.4286). All data are presented as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.