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. Author manuscript; available in PMC: 2021 Feb 24.
Published in final edited form as: Cell Rep. 2021 Feb 2;34(5):108718. doi: 10.1016/j.celrep.2021.108718

Figure 6. GNAO1 mutations impair locomotor behavior in mice.

Figure 6.

(A) Schematic of targeting expression of GNAO1 variants (G203R, R209C, or WT control) in defined striatal neurons by stereotaxic delivery of Cre-dependent AAV in either adult Drd1aCre (dMSN Gαo expression) or Drd2Cre (iMSN Gαo expression) mice.

(B) Hindlimb clasping pathology score for Drd1aCre mice expressing WT Gαo (n = 7), G203R Gαo (n = 7), or R209C Gαo (n = 7) (one-way ANOVA, Dunnett’s multiple comparisons test; WT versus G203R p = 0.0414, WT versus R209C p = 0.0050), and hindlimb clasping pathology score for Drd2Cre mice expressing WT Gαo (n = 7), G203R Gαo (n = 6), or R209C Gαo (n = 7) (one-way ANOVA, Dunnett’s multiple comparisons test; WT versus G203R p = 0.0003, WT versus R209C p = 0.0021).

(C) Latency to fall off a rotating beam while walking backward for Drd1aCre mice expressing WT Gαo (n = 7), G203R Gαo (n = 7), or R209C Gαo (n = 7) (one-way ANOVA, Dunnett’s multiple comparisons test; WT versus G203R p = 0.0019, WT versus R209C p = 0.0003), and latency to fall off a rotating beam while walking backward for Drd2Cre mice expressing WT Gαo (n = 7), G203R Gαo (n = 6), or R209C Gαo (n = 7) (one-way ANOVA, Dunnett’s multiple comparisons test; WT versus G203R p < 0.0001, WT versus R209C p < 0.0001).

(D) Ledge test pathology score for Drd1aCre mice expressing WT Gαo (n = 7), G203R Gαo (n = 7), or R209C Gαo (n = 7) (one-way ANOVA, Dunnett’s multiple comparisons test; WT versus G203R p = 0.0015, WT versus R209C p = 0.0041), and ledge test pathology score for Drd2Cre mice expressing WT Gαo (n = 7), G203R Gαo (n = 6), or R209C Gαo (n = 7) (one-way ANOVA, Dunnett’s multiple comparisons test; WT versus G203R p < 0.0001, WT versus R209C p < 0.0001).

(E) Accelerating rotarod learning rate for Drd1aCre mice expressing WT Gαo (n = 7), G203R Gαo (n = 7), or R209C Gαo (n = 7) (one-way ANOVA, Dunnett’s multiple comparisons test; WT versus G203R p = 0.6411, WT versus R209C p = 0.5076), and accelerating rotarod learning rate for Drd2Cre mice expressing WT Gαo (n = 7), G203R Gαo (n = 6), or R209C Gαo (n = 7) (one-way ANOVA, Dunnett’s multiple comparisons test; WT versus G203R p = 0.9979, WT versus R209C p = 0.9093). All data are presented as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.