Figure 4.

Low Sox2 expression is compatible with robust neuroectoderm differentiation

(A) qRT-PCR analysis of Sox2^−/− (−/−) Sox2-low, −/− Sox2-low rescue (+Sox2) and WT iPSCs in a neurectoderm monolayer differentiation assay for markers of pluripotency (Nanog) and neurectoderm (Pax6, Ascl1, and Sox1).

(B) qRT-PCR analysis of retroviral Sox2 in −/− Sox2-low iPSCs during neurectoderm monolayer differentiation.

(C) Phase images of −/− Sox2-low iPSCs and Sox2-low rescue iPSCs on day 7 of the neurectoderm monolayer differentiation assay.

Error bars indicate standard deviation of replicate qPCR reactions (n = 3).