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. 2021 Feb 18;21(4):367. doi: 10.3892/etm.2021.9798

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Copyright: © Luan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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NEAT1 represses RB cell migration, invasion and EMT via targeting miR-24-3p. Y79 and WERI-RB1 cells were treated with miR-24-3p, miR-NC, sh-NEAT1, sh-NC, sh-NEAT1 + anti NC or sh-NEAT1 + anti miR-24-3p. (A) Migrated and (B) invaded cell numbers of Y79 and WERI-RB1 cells were evaluated by Transwell assay. Protein expression levels of MMP9, N-cadherin and E-cadherin in (C) Y79 and (D) WERI-RB1 cells were detected by western blot analysis. ^*P<0.05, ^**P<0.01 and ^***P<0.001. miR, microRNA; NEAT1, nuclear paraspeckle assembly transcript 1; RB, retinoblastoma; MMP, matrix metalloproteinase; sh, short hairpin RNA; NC, negative control; EMT, epithelial-to-mesenchymal transition.