Skip to main content
. 2021 Feb 18;21(4):367. doi: 10.3892/etm.2021.9798

Figure 5.

Figure 5

NEAT1 upregulates LRG1 expression via miR-24-3p in RB cells. (A) Potential binding sites between miR-24-3p and the 3'UTR of LRG1. (B) mRNA expression levels of LRG1 in RB tissues and healthy tissues were determined via reverse transcription-quantitative PCR. ***P<0.001 vs. healthy. (C) Correlation between LRG1 and miR-24-3p was determined using Pearson's correlation analysis. (D) Protein expression levels of LRG1 in RB tissues and healthy tissues were detected via western blot analysis. ***P<0.001 vs. healthy. Luciferase activities were detected in (E) Y79 and (F) WERI-RB1 cells co-transfected with LRG1 3'UTR-WT or LRG1 3'UTR-MUT and miR-NC or miR-24-3p. ***P<0.001 vs. miR-NC. Protein expression levels of LRG1 in (G) Y79 and (H) WERI-RB1 cells treated with miR-24-3p, miR-24-3p + pcDNA-NEAT1 or the corresponding controls were measured via western blotting ***P<0.001 vs. miR-NC, **P<0.01 and ***P<0.001 vs. miR-24-3p+pcDNA. (I) Protein expression level of LRG1 in ARPE-19, Y79 and WERI-RB1 cells was detected via western blotting. ***P<0.001 vs. ARPE-19. (J) LRG1 protein expression in Y79 and WERI-RB1 cells transfected with sh-NC or sh-LRG1. ***P<0.001 vs. sh-NC. (K) Migratory and (L) invasive abilities of Y79 and WERI-RB1 cells were evaluated via Transwell assays. **P<0.01 and ***P<0.001 vs. sh-NC. miR, microRNA; NEAT1, nuclear paraspeckle assembly transcript 1; RB, retinoblastoma; sh, short hairpin RNA; NC, negative control; WT, wild-type; MUT, mutant; LRG1, leucine-rich α-2-glycoprotein; UTR, untranslated region.