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. 2021 Feb 18;21(4):367. doi: 10.3892/etm.2021.9798

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Copyright: © Luan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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NEAT1 affects RB cell migration, invasion and EMT by upregulating LRG1 expression via the sponging of miR-24-3p. Y79 and WERI-RB1 cells were divided into six groups and transfected with the following: pcDNA-LRG1, pcDNA, pcDNA-LRG1 + miR-24-3p, pcDNA-LRG1 + miR-NC, pcDNA-LRG1 + miR-24-3p + pcDNA-NEAT1 or pcDNA-LRG1 + miR-24-3p + pcDNA. (A) Migration and (B) invasion of Y79 and WERI-RB1 cells were evaluated via a Transwell assay. MMP9, N-cadherin and E-cadherin protein levels in (C) Y79 and (D) WERI-RB1 cells were determined via a western blot assay. ^*P<0.05, ^**P<0.01 and ^***P<0.001. miR, microRNA; NEAT1, nuclear paraspeckle assembly transcript 1; RB, retinoblastoma; MMP, matrix metalloproteinase; sh, short hairpin RNA; NC, negative control; EMT, epithelial-to-mesenchymal transition; LRG1, leucine-rich α-2-glycoprotein.