Figure 3.

mRNA expression of MSK1, IL-1β, TNF-α in the brain cortex at various survival time intervals after SAH. The quantitative analysis demonstrated the high expression of MSK1 mRNA in the control and sham groups. The level of MSK1 decreased gradually after SAH, then peaked at 3 days, and was increased during the following days in the cortex. Conversely, analysis of the levels of IL-1β and TNF-α mRNA indicated that these mRNA gradually increased following SAH, reaching peak levels at 1 day, and then gradually decreased during the following days in the cortex. The correlation was determined by Spearman's analysis. Bars represent the mean ± SEM (n=6 in each group). Statistical analysis was performed using one-way analysis of variance followed by Dunnett's post-hoc test. ^*P<0.05 and ^**P<0.01 vs. sham and normal groups. MSK1, mitogen and stress-activated protein kinase 1; IL-1β, interleukin-1β; TNF-α, tumor necrosis factor-α; SAH, subarachnoid hemorrhage.