Figure 2.

DEC1 is upregulated in OC cell lines. Expression of DEC1 in OC cell lines SKOV3 and OVCAR3 and ovarian epithelial cell line (ISOE80) was detected using (A) western blotting and (B) reverse transcription-quantitative PCR. (C) Expression of DEC1 in SKOV3 and OVCAR3 cells transfected with NC shRNA or shDEC1 was evaluated using western blotting. ^*P<0.05, ^**P<0.01 vs. ISO80 cells or control (NC) group. NC, negative control; shRNA, short hairpin RNA; DEC1, differential embryo-chondrocyte expressed gene 1; OC, ovarian cancer.