Figure 5.

Continued. Knockdown of DEC1 inhibits migration and invasion of ovarian cancer cells. Wound healing assay was performed to evaluate the effects of DEC1 on migration in (A) SKOV3 and (B) OVCAR3 cells. Scale bar, 300 µm. Transwell invasion assay was performed to evaluate the effects of DEC1 on invasion in (C) SKOV3 and (D) OVCAR3 cells. Scale bars, 300 µm. Western blotting was conducted to evaluate the effects of DEC1 on the epithelial-mesenchymal transition-related proteins in (E) SKOV3 and (F) OVCAR3 cells. ^**P<0.01 vs. control (NC) group. NC, negative control; shRNA, short hairpin RNA; DEC1, differential embryo-chondrocyte expressed gene 1; α-SMA, α-smooth muscle actin.