REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal cathepsin B	Santa Cruz Biotechnology	Cat#sc-365558
Mouse monoclonal β-actin	Santa Cruz Biotechnology	Cat#sc-47778
Chemicals, peptides, and recombinant proteins
Acetic acid, glacial	Samchun chemicals	Cat#A0051
Acetonitrile	Avantor J.T. Backer	Cat#901203
Acridine orange	Immunochemistry Technologies	Cat#6130
4-(Aminomethyl)benzoic acid	Alfa Aesar	Cat#B23519
Ammonium acetate	Sigma-Aldrich	Cat#A7262
Apoptozole	This article	N/A
3,5-Bis(trifluoromethyl)benzaldehyde	Tokyo Chemical Industry	Cat#B1751
Bromophenol blue	Sigma-Aldrich	Cat#B5525
tert-Butyl 2-[2-(2-aminoethoxy)ethoxy] ethylcarbamate	Sigma-Aldrich	Cat#89761
Chloroform-D (CDCl3)	Cambridge Isotope Laboratory	Cat#DLM-7
Compound 1	This article	N/A
Compound 2	This article	N/A
Dichloromethane	Duksan Pure Chemicals	N/A
Digitonin	Sigma-Aldrich	Cat#D141
4,4′-Dimethoxybenzil	Tokyo Chemical Industry	Cat#A1028
4-Dimethylaminopyridine (DMAP)	Alfa Aesar	Cat#A13016
N,N-Dimethylformamide (DMF)	Duksan Pure Chemicals	N/A
Dimethylsulfoxide (DMSO)	VWR Life Science	Cat#67-68-5
Dulbecco’s modified Eagle’s medium (DMEM)	Thermo Fisher Scientific	Cat#11995-073
Dulbecco’s phosphate buffered saline (DPBS)	Welgene	Cat#LB-001-02
Ethyl acetate	Duksan Pure Chemicals	N/A
1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC)	Glentham Life Sciences	Cat#GP0849
Ethylenediaminetetraacetic acid (EDTA)	Sigma-Aldrich	Cat#EDS
Ethyleneglycolbistetraacetic acid (EGTA)	Sigma-Aldrich	Cat#324626
Fetal bovine serum (FBS)	Thermo Fisher Scientific	Cat#26140079
Glycerol	Amresco	Cat#0942C411
HEPES	Sigma-Aldrich	Cat#H3375
Hexane	Duksan Pure Chemicals	N/A
Magnesium chloride (MgCl2)	Sigma-Aldrich	Cat#M1028
β-Mercaptoethanol	Sigma-Aldrich	Cat#M3148
Mounting solution	Sigma-Aldrich	Cat#F4680
Nitrogen gas	Dong-A specialty gases	N/A
PBS	Intron	Cat#BP007A
Pefabloc	Sigma-Aldrich	Cat#11429868001
Penicillin/streptomycin solution	Thermo Fisher Scientific	Cat#15140122
Polyvinylidene fluoride (PVDF) membrane	Merck Millipore	Cat#IPVH00010
Potassium chloride (KCl)	Sigma-Aldrich	Cat#P9541
Protease inhibitor cocktail	Roche	Cat#11836153001
Silica gel 60 (0.040–0.063 mm)	Merck Millipore	Cat#109385
Sodium acetate	Sigma-Aldrich	Cat#S2839
Sodium bicarbonate (NaHCO3)	Samchun Chemicals	Cat#S0343
Sodium chloride (NaCl)	Samchun Chemicals	Cat#S2097
Sodium deoxycholate	Sigma-Aldrich	Cat#D6750
Sodium dodecyl sulfate (SDS)	Intron	Cat#BS003
Sodium sulfate (Na2SO4), anhydrous	Junsei	Cat#83465S1250
Sucrose	Sigma-Aldrich	Cat#S0389
Syringe filter	Cole-Parmer	Cat#EW-81054-42
TLC silica gel 60 F254	Merck Millipore	Cat#105715
Trichloroacetic acid	Sigma-Aldrich	Cat#T6399
Trifluoroacetic acid (TFA)	Daejung Chemicals	Cat#8558-4400
Tris	Biopure	Cat#TRS001
Trypsin-EDTA	Sigma-Aldrich	Cat#T4174
Tween 20	Amresco	Cat#2283C019
West-ZOL plus	Intron	Cat#16021
Other
Buchner funnel	N/A	N/A
Column	Daihan Scientific	N/A
Gas tight syringe 10 mL	Hamilton	Cat#4015-54010
Heating mantle	Daihan Scientific	Cat#DH.WHM12113
Magnetic stirrer	Daihan Scientific	Cat#DH.WMH03120
Magnetic stirring bar	Daihan Scientific	Cat#CW.001.620
Reflux condenser	N/A	N/A
Rotary evaporator	EYELA	Cat#N-1200A
25 mL round-bottom flask	Daihan Scientific	Cat#SL.Fla2176
50 mL round-bottom flask	Daihan Scientific	Cat#SL.Fla2178
Rubber balloon	Neotex	N/A
Rubber septum	Sigma-Aldrich	Cat#Z553964
Separatory funnel 250 mL	Daihan Scientific	Cat#GL.149.209.04
Separatory funnel 500 mL	Daihan Scientific	Cat#GL.149.209.05
100 mL two-neck round-bottom flask	Daihan Scientific	Cat#SL.Fla2213
Critical commercial assays
Lysosome isolation kit	Abcam	Cat#ab234047
Experimental models: cell lines
HeLa cervical cancer cells	Korea Cell Line Bank	Cat#10002
Software and algorithms
ZEN 2011	Zeiss microscopy	https://www.zeiss.com/microscopy/int/downloads/zen.html