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Generation of PDOXs
Steps and key requirement for establishing mouse PDOXs from PDO 3D cultures. The steps include dissociation of PDOs, labeling with lentiviral fluorescent reporters, engrafting glioma in mouse brains, detecting PDOXs using bioluminescence imaging (BLI) and MRI imaging, and molecular assays. Ctl, control mice are compared to PDOX mice.
