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. 2021 Feb 18;2(1):100345. doi: 10.1016/j.xpro.2021.100345

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© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Drug synergy assays in PDOs

Steps 1–4 show the utilization of PDOs to determine the antitumor activity of drugs A and B. Drugs are added to the media from a 10× stock to reach 1× final concentration. Treatment is continued for 3 days, based on initial determination of GI₅₀ concentration In some cases, treatment may be continued for 7 days to see the full inhibitory effects. Effects on PDO cell viability is determined by assessing intracellular ATP levels using CellTiter-Glo (Promega) following the manufacturer’s instructions. Data are input into SynergyFinder software to determine synergy between drugs A and B.