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. 2021 Feb 18;2(1):100345. doi: 10.1016/j.xpro.2021.100345

Table 1.

Cycling parameters for subtyping of GBM from PDOs, PDOXs, and original tumor

Stage 1 Stage 2 Stage 3
Melt Curve
Step 1 Step 2 Step 1 Step 2 Step 3
50°C 95°C 95°C 60°C 95°C 60°C 95°C
2:00 min 2:00 min 15 s 1:00 min 15 s 1:00 min 15 s

Stage 1 = 1×, Stage 2 = 1×, Stage 3 = 40×