Table 1.
Stage 1 | Stage 2 | Stage 3 |
Melt Curve |
|||
---|---|---|---|---|---|---|
Step 1 | Step 2 | Step 1 | Step 2 | Step 3 | ||
50°C | 95°C | 95°C | 60°C | 95°C | 60°C | 95°C |
2:00 min | 2:00 min | 15 s | 1:00 min | 15 s | 1:00 min | 15 s |
Stage 1 = 1×, Stage 2 = 1×, Stage 3 = 40×
Stage 1 | Stage 2 | Stage 3 |
Melt Curve |
|||
---|---|---|---|---|---|---|
Step 1 | Step 2 | Step 1 | Step 2 | Step 3 | ||
50°C | 95°C | 95°C | 60°C | 95°C | 60°C | 95°C |
2:00 min | 2:00 min | 15 s | 1:00 min | 15 s | 1:00 min | 15 s |
Stage 1 = 1×, Stage 2 = 1×, Stage 3 = 40×