Table 2.
Dose response assays to assess synergy between drug A (e.g., BEZ235 (BEZ)) and drug B (e.g., temozolomide (TMZ))
| Tested drug A concentration | BEZ 0.1 μM | BEZ 0.25 μM | BEZ 0.5 μM | BEZ 1 μM | |
|---|---|---|---|---|---|
| TMZ 0 BEZ 0 |
TMZ 0 BEZ 1 μM |
TMZ 0 BEZ 2.5 μM |
TMZ 0 BEZ 5 μM |
TMZ 0 BEZ 10 μM |
Tested drug B concentration |
| TMZ 10 μM BEZ 0 |
TMZ 10 μM BEZ 1 μM |
TMZ 10 μM BEZ 2.5 μM |
TMZ 10 μM BEZ 5 μM |
TMZ 10 μM BEZ 10 μM |
TMZ 1 μM |
| TMZ 100 μM BEZ 0 |
TMZ 100 μM BEZ 1 μM |
TMZ 100 μM BEZ 2.5 μM |
TMZ 100 μM BEZ 5 μM |
TMZ 100 μM BEZ 10 μM |
TMZ 10 μM |
| TMZ 250 μM BEZ 0 |
TMZ 250 μM BEZ 1 μM |
TMZ 250 μM BEZ 2.5 μM |
TMZ 250 μM BEZ 5 μM |
TMZ 250 μM BEZ 10 μM |
TMZ 25 μM |
| TMZ 500 μM BEZ 0 |
TMZ 500 μM BEZ 1 μM |
TMZ 500 μM BEZ 2.5 μM |
TMZ 500 μM BEZ 5 μM |
TMZ 500 μM BEZ 10 μM |
TMZ 50 μM |
| TMZ 1 mM BEZ 0 |
TMZ 1 mM BEZ 1 μM |
TMZ 1 mM BEZ 2.5 μM |
TMZ 1 mM BEZ 5 μM |
TMZ 1 mM BEZ 10 μM |
TMZ 100 μM |
TMZ or BEZ are indicated as 10× concentrations and used to achieve final 1× concentrations.