| Reagent or resource | Final concentration | Volume |
|---|---|---|
| EMEM | - | 490 mL |
| Glutamax (100×) | 1× (2 mM of L-alanyl-L-glutamine dipeptide) | 5 mL |
| Penicillin/streptomycin (100×) | 100 U/mL of penicillin and 100 μg/mL of streptomycin | 5 mL |
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