REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
BMI1 antibody (1:200)	Cell Signaling Technology	6964
GALC antibody (1:200)	Santa Cruz Biotechnology	SC-67352
GFAP antibody (1:300)	Cell Signaling Technology	3670
NESTIN antibody (1:200)	Millipore	MAB5326
SOX2 antibody (1:400)	Cell Signaling Technology	23064
TLX antibody (5 μg/mL)	Invitrogen	PA5-40484
TUJ1 antibody (1:200)	Millipore	MAB1637
P16 antibody (1:1,000)	Santa Cruz Biotechnology	SC-81157
Nucleolin antibody (1–2 μg/mL)	Abcam	Ab-22758
Phospho-S6 antibody (1:1,000)	Cell Signaling Technology	2215S
Phalloidin (1:400)	Thermo Fisher Scientific	A12380
DAPI (4′,6-diamidino-2-phenylindole, dilactate) (300 nM)	BioLegend	422801
Chemicals, peptides, and recombinant proteins
GlutaMAX-I	Gibco	35050-061
Advanced DMEM/F12	Gibco	12634-010
Eagle’s minimum essential medium (EMEM)	ATCC	30-2003
Matrigel matrix basement membrane	Corning	356237
B27 Supplement without vitamin A	Gibco	12587-010
Recombinant human EGF	Peprotech	AF-100-15
Recombinant human FGF-basic (BFGF)	Peprotech	AF-100-18B
Hydrocortisone stock solution (200×)	STEMCELL Technologies	07925
Penicillin and streptomycin (10,000 U/mL)	Gibco	15140-122
Sodium pyruvate	Himedia	TCL015-100mL
Primocin	InvivoGen	Ant-pm-1
Accutase	STEMCELL	07920
37% Formaldehyde	Fisher Bioreagents	BP-531-500
Phosphate buffer saline (PBS) without calcium and magnesium	Lonza	17-516F
Triton X-100	Sigma-Aldrich	X100-100mL
Ketamine	Henry Schein	NDC 11695-0702-1
Xylazine	Akom	NDC 59399-110-20
Minimal essential medium (MEM)	Gibco	11090-081
Fetal bovine serum (FBS)	Sigma-Aldrich	F4135-500 mL
Bovine serum albumin (BSA), Fraction V	Sigma-Aldrich	2930-100GM
Cell recovery solution (CRS)	Corning	354253
Betadine	Fisher Scientific	19027132
Hydrogen peroxide (3%)	Sigma-Aldrich	88597-100mL
Drugs for testing and screening	Local pharmacy or commercial sources (e.g., Selleck)	N/A
Critical commercial assays
RNeasy Kit	Qiagen	75162
SuperScript IV VILO MasterMix	Invitrogen	11756050
SYBR Green Master Mix	Thermo Fisher Scientific	A25742
CellTiter-Glo Assay	Promega	G7572
Biological samples
Surgical specimen of tumor removed from GBM patients	Surgical facility	N/A
Experimental models: organisms/strains
6- to 12-week-old NSG mice	The Jackson Laboratory	NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ
Other
Ultra-low attachment 96-well plates	Grenier	655185
Ultra-low attachment 384-well plates	Grenier	781186
Falcon 40 μm cell strainer	Corning	352340
IncuCyte HD system	Sartorius	N/A
Nikon A1R Si confocal microscope	Nikon	N/A
IVIS Spectrum imaging station	PerkinElmer	N/A
StepOne plus qPCR instrument	Applied Biosystems	4376600
Victor3 multilabel plate reader	PerkinElmer	1420-011
Stereotactic apparatus	Stoelting	51901
Stereotactic probe holder attachment	Stoelting	51681
Micromotor high-speed drill with drill bits	Stoelting	51449
Drill bit, 45 mm	Stoelting	514551
10 μL syringe, Model 701	Hamilton	7635-01
30 gauge removable needle	Hamilton	7803-07
Rectal probe	Stoelting	5060-040
Vetbond tissue adhesive	3M	1469SB
Bonewax	Ethicon	W31G
Microdrill	Foredom	MH-170
Micromotor control	Foredom	HP4-917
Foot speed control	Foredom	HP4-970
Homeothermic monitoring system	Harvard Apparatus	55-7020
Remote infuse/withdraw PHD ULTRA Nanomite programmable syringe pump	Harvard Apparatus	70-3601
Pura lube ophthalmic ointment	Dechra	NDC 17033-211-040
Veet hair removal cream	Any commercial source (e.g., Amazon)	N/A
Syringe (1 mL)	BD	309659
Syringe needle (27 gauge)	BD	305109
Scalpels	BD	371610
Sodium chloride (0.9%)	Hospira	NDC 0509-4888-06
Software and algorithms
ELDA software	Walter + Eliza Hall	http://bioinf.wehi.edu.au/software/elda/
TCGA database	NHGRI & NCI	https://portal.gdc.cancer.gov/
Prism 8	GraphPad	https://www.graphpad.com/scientific-software/prism/
Drug synergy software	Synergy Finder	https://synergyfinder.fimm.fi
Image analysis software	NIH	https://imagej.nih.gov/ij/
StepOne software	Applied Biosystems	N/A
IVIS imaging software	PerkinElmer	N/A