Additional file 19: Video 5. Time-resolved 3D volume rendering of a growing liver organoid with cell segmentation and tracking. hCCAOs expressing H2B-eGFP as nuclei marker (red) and LifeAct-mCherry as F-actin cytoskeletal marker (green) were recorded in a Z1-FEP-cuvette for a total of 132 h. The movie shows data recorded between 84 and 108 h. Red spheres illustrate tracked cell nuclei and rainbow-coloured lines indicate the travelled tracks (colour code: red to blue – timepoint 84 to 108). Single as well as multiple organoid tracking is shown. Rotation as well as uni-directional cell movements are visible. Microscope: Zeiss Lightsheet Z.1; detection objective: W Plan-Apochromat 20x/1.0, illumination objective: Zeiss LSFM 10x/0.2; laser lines: 488 nm, 561 nm; filters: laser block filter (LBF) 405/488/561; voxel size: 1.02 × 1.02 × 2.00 μm3; recording interval: 30 min; 3D rendering and tracking software: Arivis Vision4D. (MP4 36,759 kb)
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