Additional file 20: Video 6. Alterations in rotation velocity of neighbouring organoids. Video shows two mPOs as an excerpt from an entire culture grown within one Z1-FEP-cuvette. The organoids expressed Rosa26-nTnG (grey) as nuclei marker and were imaged over 20 h. Besides the differences in nuclei size, both organoids display different behaviour. Cell tracking revealed a rotational motion of the epithelial cell monolayer of the organoid with the small roundish nuclei and no rotational motion of the organoid with the big, elongated cell nuclei. The two organoids are in close contact but do not fuse or interact with each other. Microscope: Zeiss Lightsheet Z.1; detection objective: W Plan-Apochromat 20x/1.0, illumination objective: Zeiss LSFM 10x/0.2; laser lines: 488 nm, 561 nm; filters: laser block filter (LBF) 405/488/561; voxel size: 1.02 × 1.02 × 2.00 μm3; recording interval: 30 min; 3D rendering and segmentation software: Arivis Vision4D. (MP4 61,855 kb)
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