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. 2021 Feb 23;9:55. doi: 10.1186/s40168-020-00981-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Validation of sProtein identification with synthetic peptides. a The novel sProtein BT6 (57 aa) predicted by Prodigal and detected with the unique, fully tryptic peptide RSQLEHEVSVAQER is shown, as well as b the MS/MS spectrum which was assigned to the peptide spectrum match (PSM) by the search algorithm. c The unique peptide was synthesized and a MS/MS spectrum was generated. d Both spectra were compared using NIST’s MS search software, which resulted in a match factor of 814 and reverse match factor of 924, well above the cut-off values, thereby confirming expression of the novel sProtein BT6