VSTM2A expression in MTSCC by RNA in situ hybridization (ISH). (A, C) Classic MTSCC with elongated tubules in a myxoid matrix (H&E, 40X and 200X, respectively). (B, D) VSTM2A expression in MTSCC by RNA ISH (40X and 200X, respectively), with brown, punctate dots and no background staining. (E, F) Negative expression of VSTM2A in renal cortex and medulla, respectively (400X); inset: H&E (400X).