Figure 3.

Entinostat attenuated proinflammatory response in vitro. For in vitro studies, Raw 264.7cells, a macrophage-like cell line, were pre-treated with Entinostat (0.25μM or 0.5 μM) for 1 hour and that was followed by an 18-hour co-treatment with hemin (30 μM) and the supernatant was collected. The level of proinflammatory cytokines, TNF-α (A), and IL-6 (B), were quantified using ELISA, as detailed in methods. Both TNF-α and IL-6 levels were significantly reduced with Entinostat treatment in comparison to respective control. (* p < 0.05, ** p <0.01, ***p<0.001 vs. hemin alone (control; n=4, hemin; n=8 and hemin +Entinostat; n=4).