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. Author manuscript; available in PMC: 2021 Feb 24.
Published in final edited form as: Methods Enzymol. 2018 Sep 27;615:285–332. doi: 10.1016/bs.mie.2018.08.017

Fig. 1.

Fig. 1

(A) 1H and 15N resonances of the Lys and Arg side chains of the HoxD9 homeodomain bound to 24-bp specific DNA at 35°C. (B) Effect of D2O in the sample buffer. D2O for NMR lock should separately be sealed to avoid exchange between isotopically different species (i.e., NH3+, NDH2+, ND2H+). (C) Two types of coaxial NMR tubes for separating the sample and D2O.