KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-gp120 (HIV-1, Pan) | Immune Technology | Cat# IT-001-Pan-M1 |
| Anti-gp120 (SIVmac239, 3.11H) | Termini et al., 2017a | N/A |
| HRP-goat anti-mouse IgG (H+L) | Immune Technology | Cat# IT-200-010 |
| HRP-goat anti-rhesus IgG (H+L) | Southern Biotech | Cat# 6200-05 |
| 10-1074 | NIH AIDS Reagent Program | Cat# 12477 |
| PGT121 | NIH AIDS Reagent Program | Cat# 12343 |
| PGT128 | NIH AIDS Reagent Program | Cat# 13352 |
| Bacterial and Virus Strains | ||
| MAX Efficiency Stbl2 competent cells | Invitrogen | Cat# 10268019 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Artocarpus integrifolia (jacalin) HRP | United States Biological | Cat# A3590-11 |
| Unconjugated jacalin | Vector Laboratories | Cat# L-1150-10 |
| Critical Commercial Assays | ||
| Britelite Plus reporter gene assay system | Perkin Elmer | Cat# 6066766 |
| HIV p24 antigen capture assay | Advanced Bioscience Laboratories, Inc. | Cat# 5447 |
| SIV p27 antigen capture assay | Advanced Bioscience Laboratories, Inc. | Cat# 5436 |
| Experimental Models: Cell Lines | ||
| HEK293T cells | ATCC | Cat# CRL-3216 |
| HEK293T gale knockout cells | Termini et al., 2017b | N/A |
| HEK293T gale & galk2 knockout cells | Termini et al., 2017b | N/A |
| TZM-bl cells | NIH AIDS Reagent Program | Cat# 8129 |
| Recombinant DNA | ||
| NL4-3 | NIH AIDS Reagent Program | Cat# 114 |
| NL-AD8 | NIH AIDS Reagent Program | Cat# 11346 |
| NL-VA40774 | Silver et al., 2019 | N/A |
| Recombinant NL4-3 virus (NL-HM215335, NL-KX693469, NL-GU329473) | This paper | N/A |
| gp120 expression cassettes (samples #1-37) | This paper | N/A |
| Software and Algorithms | ||
| GraphPad Prism | https://www.graphpad.com | RRID: SCR_002798 |
| MacVector | https://macvector.com | RRID: SCR_015700 |
| SnapGene | https://www.snapgene.com | RRID: SCR_015052 |
| GlycoWorkbench | https://code.google.com/archive/p/glycoworkbench/ | RRID: SCR_000782 |
| Python algorithm for analyzing V1 length and NetOGlyc4.0 score | This paper | N/A |
| Other | ||
| jetPRIME | Polyplus-transfection S.A. | Cat# 114-15 |
| BIO-MPM-1 serum-free medium | Biological Industries USA | Cat# 05-060-1A |
| HisPur Ni-NTA spin columns, 3 mL | ThermoFisher Scientific | Cat# 88226 |
| Laemmli sample buffer | Sigma-Aldrich | Cat# S3401 |
| iBlot 2 Gel Transfer Device | ThermoFisher Scientific | Cat# IB21001 |
| iBind Western Device | ThermoFisher Scientific | Cat# SLF1000 |