Table 3. Inter- and intra-day performance.
Inter-day mean ± standard deviation (Std Dev), accuracy (% error), and precision (% CV) were determined for 208 blinded PT samples for 5 LF levels analyzed over 17 days for both the 4-h and ON methods (A). For blinded samples analyzed 3 or more times a day, the intra-day means, % error and % CV were determined daily, then mean ± Std Dev, for the daily results were determined (B).
A. Inter-Day Precision and Accuracy (17 Days Total) | |||||
---|---|---|---|---|---|
LF PT (ng/mL) | 0.721 | 0.383 | 0.204 | 0.108 | 0.058 |
N | 40 | 43 | 41 | 42 | 42 |
Fast (4-h) Inter-Day | |||||
Mean ± Std Dev | 0.708 ± 0.072 | 0.380 ± 0.039 | 0.197 ± 0.021 | 0.107 ± 0.014 | 0.058 ± 0.014 |
% CV | 10.2 | 10.3 | 10.6 | 13.1 | 23.6 |
% error | 1.9 | 0.72 | 3.5 | 1.3 | 0.2 |
Overnight Inter-Day | |||||
Mean ± Std Dev | 0.661 ± 0.072 | 0.345 ± 0.037 | 0.184 ± 0.017 | 0.102 ± 0.010 | 0.056 ± 0.0047 |
% CV | 11.0 | 10.7 | 9.0 | 9.9 | 8.5 |
% error | 8.5 | 9.8 | 9.6 | 5.1 | 3.8 |
B. Intra-Day Precision and Accuracy | |||||
LF PT (ng/mL) | 0.721 | 0.383 | 0.204 | 0.108 | 0.058 |
N Days | 5 | 8 | 8 | 9 | 9 |
N Samples | 17 | 25 | 24 | 27 | 27 |
Fast (4-h) Intra-Day | |||||
Mean ± Std Dev | 0.693 ± 0.047 | 0.383 ± 0.024 | 0.190 ± 0.033 | 0.104 ± 0.21 | 0.058 ± 0.015 |
% CV | 4.2 ± 3.1 | 6.0 ± 3.8 | 7.5 ± 3.8 | 7.1 ± 4.2 | 10.7 ± 9.0 |
% error | 3.9 ± 6.5 | 5.5 ± 2.3 | 7.5 ± 5.1 | 6.9 ± 8.4 | 10.8 ± 18.2 |
Overnight Intra-Day | |||||
Mean ± Std Dev | 0.649 ± 0.032 | 0.342 ± 0.031 | 0.187 ± 0.012 | 0.104 ± 0.007 | 0.056 ± 0.003 |
% CV | 5.1 ± 3.0 | 6.4 ± 2.5 | 8.0 ± 4.4 | 6.3 ± 7.0 | 5.3 ± 5.7 |
% error | 10.0 ± 4.5 | 10.6 ± 8.0 | 8.7 ± 5.3 | 3.6 ± 7.2 | 3.0 ± 6.0 |