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. Author manuscript; available in PMC: 2021 Feb 24.
Published in final edited form as: Anal Biochem. 2017 Dec 7;543:97–107. doi: 10.1016/j.ab.2017.12.007

Table 3. Inter- and intra-day performance.

Inter-day mean ± standard deviation (Std Dev), accuracy (% error), and precision (% CV) were determined for 208 blinded PT samples for 5 LF levels analyzed over 17 days for both the 4-h and ON methods (A). For blinded samples analyzed 3 or more times a day, the intra-day means, % error and % CV were determined daily, then mean ± Std Dev, for the daily results were determined (B).

A. Inter-Day Precision and Accuracy (17 Days Total)
LF PT (ng/mL) 0.721 0.383 0.204 0.108 0.058
N 40 43 41 42 42
Fast (4-h) Inter-Day
Mean ± Std Dev 0.708 ± 0.072 0.380 ± 0.039 0.197 ± 0.021 0.107 ± 0.014 0.058 ± 0.014
% CV 10.2 10.3 10.6 13.1 23.6
% error 1.9 0.72 3.5 1.3 0.2
Overnight Inter-Day
Mean ± Std Dev 0.661 ± 0.072 0.345 ± 0.037 0.184 ± 0.017 0.102 ± 0.010 0.056 ± 0.0047
% CV 11.0 10.7 9.0 9.9 8.5
% error 8.5 9.8 9.6 5.1 3.8
B. Intra-Day Precision and Accuracy
LF PT (ng/mL) 0.721 0.383 0.204 0.108 0.058
N Days 5 8 8 9 9
N Samples 17 25 24 27 27
Fast (4-h) Intra-Day
Mean ± Std Dev 0.693 ± 0.047 0.383 ± 0.024 0.190 ± 0.033 0.104 ± 0.21 0.058 ± 0.015
% CV 4.2 ± 3.1 6.0 ± 3.8 7.5 ± 3.8 7.1 ± 4.2 10.7 ± 9.0
% error 3.9 ± 6.5 5.5 ± 2.3 7.5 ± 5.1 6.9 ± 8.4 10.8 ± 18.2
Overnight Intra-Day
Mean ± Std Dev 0.649 ± 0.032 0.342 ± 0.031 0.187 ± 0.012 0.104 ± 0.007 0.056 ± 0.003
% CV 5.1 ± 3.0 6.4 ± 2.5 8.0 ± 4.4 6.3 ± 7.0 5.3 ± 5.7
% error 10.0 ± 4.5 10.6 ± 8.0 8.7 ± 5.3 3.6 ± 7.2 3.0 ± 6.0