FIGURE 4.

A, An example DENSE circumferential strain map was used to identify infarcted and noninfarcted remote regions of the myocardium in post-MI mice, and these regions were applied to nitroxide-enhanced images acquired at matched locations. B, Example vascular (C_V) and 2CXRM remote zone and infarct zone C_T fits in a mouse 1 day after infarction. C, Time courses of mean K_red for infarct and remote regions from mice before and for 21 days after MI demonstrate the rise and decline of oxidative stress in the infarct region. D, Example confocal images of DHE-stained myocardium obtained on Day 1 after MI. Specifically, overlays of 488 nm (for oxidation products of DHE) and 405 nm (for DHE) are shown for remote and infarct regions. E, DHE fluorescence in the infarct zone is elevated compared with the remote zone in 1 day post-MI mice, indicative of oxidative stress in infarcted myocardium. In contrast, in control mice DHE fluorescence was equivalent in the septal and lateral walls. *p < 0.05 compared with baseline and Day 21 infarct, and Day 1 remote using a two-way ANOVA. **p < 0.05 compared with baseline and Day 21 infarct, and Day 4 remote using a two-way ANOVA. ^$p < 0.05 compared with control using a log ratio t test