FIG. 4.

Effect of TCDD on expression of collagen type II (COL2a) immunoreactivity in medaka hypurals. Embryos were exposed to DMSO (A) or 1 ppb TCDD (B) at 4 hpf for 1 h, washed, and incubated in ERM at 26°C until 10 dpf, at which time, five embryos per group were dechorionated and fixed for WIHC with anti-type II collagen, monoclonal antibody at 1:100. Figures are representative of the five individuals in each group. Brown color indicates positive COL2a immunoreactivity in hypurals. Bar = 100 μm.