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. 2021 Feb 24;16(2):e0247652. doi: 10.1371/journal.pone.0247652

Fig 4. Harmine induces a dose-dependent degradation of Twist1 in breast cancer cells.

Fig 4

A, The BT549 Twist+ cells were either untreated (UT), treated with DMSO, or treated with harmine at the concentrations indicated for 48 hr. Cell lysates were analyzed by blotting for Twist1 or β-actin (loading control). B, Quantitative analysis of result shown in A. C, The 4T1 Twist+ cells were either untreated (UT), treated with DMSO, or treated with harmine at the concentrations indicated for 48 hr. Cell lysates were analyzed by blotting for Twist1 or β-actin (loading control). D, Quantitative analysis of result shown in C. Definitions of statistical significance are * p < .05, ** p < .01, *** p < .001, and **** p < .0001.