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. 2021 Feb 24;7(9):eabb0737. doi: 10.1126/sciadv.abb0737

Fig. 4. miR-330-3p targets junctional adhesion molecule 2 for the maintenance of mesenchymal identity of ovarian cancer.

Fig. 4

(A) Venn diagram showing the most possible up-regulated genes targeted by plasma cell exosome–containing mir-330-3p. (B) Univariate regression analysis of the six overlapped target genes associated with ovarian cancer patient survival (Bonome dataset, n = 182). (C) mRNA level of JAM2 in COV318 and OVCAR-3 cells with respective treatment (n = 3 to 4 for each group). (D) Western blotting analysis of JAM2 protein levels in COV318 and OVCAR-3 cells with respective treatment (n = 3 for each group). (E) mRNA levels of JAM2 and EMT markers in miR-330-3p mimic–transfected or control COV318 and OVCAR-3 cells (n = 3 to 4 for each group). (F) Western blotting analysis of JAM2 and EMT markers in miR-330-3p mimic–transfected or control COV318 and OVCAR-3 cells (n = 3 for each group). (G) Wound healing analysis to assess the migration ability of COV318 and OVCAR-3 cells with respective treatment (n = 3 to 5 for each group). (H) Western blotting analysis of JAM2 and EMT markers in COV318 and OVCAR-3 cells with respective treatment (n = 3 for each group). si, small interfering. (I) mRNA levels of JAM2 in COV318 and OVCAR-3 cells with respective treatment (n = 3 to 4 for each group). (J) Transwell chamber analysis to assess the migration ability of COV318 and OVCAR-3 cells with respective treatment (n = 3 to 5 for each group). (K) Wound healing analysis to assess the migration ability of COV318 and OVCAR-3 cells with respective treatment (n = 3 to 5 for each group). Data are shown as mean ± SEM. All statistical significance was determined by a two-tailed, unpaired Student’s t test. *P < 0.05; **P < 0.01; ***P < 0.001.