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. Author manuscript; available in PMC: 2022 Jan 21.
Published in final edited form as: Mol Cell. 2020 Dec 8;81(2):281–292.e8. doi: 10.1016/j.molcel.2020.11.013

Figure 1. Formation and Isolation of the Pre-Termination Complexes (PTCs).

Figure 1.

(A) Schematic of the nucleic acid scaffold used to assemble the tertiary elongation complexes (EC18 and EC60). ntDNA - nontemplate DNA strand; tDNA - template DNA strand; nt - nucleotide; partial RUT81 – the first 40 nucleotides of canonical 81-nucleotides Rho-utilization site.

(B) Size-exclusion chromatography (SEC) of PTC60 (left panel). Red line indicates the peak fractions that were analyzed by SDS-PAGE (middle panel). Right panel shows the dynamic light scattering (DLS) analysis of the peak fraction from the SEC purification of PTC60 (%Pd, the polydispersity statistics; MW-R, estimated molecular weight).

(C) SEC of PTC18 (left panel). Red line indicates the peak fractions that were analyzed by SDS-PAGE (middle panel). Right panel shows DLS of the peak fraction from the SEC purification of PTC18.