Figure 5.

Expression of related marker proteins and genes under normoxic and hypoxic conditions by Western blotting. (a) Beclin-1 was measured by immunoblotting under normoxia and hypoxia. (b) Caspase-3 was measured by immunoblotting when incubated with HIF-1α inhibitor YC-1. The results indicate that hypoxia might induce cell chemoresistance through the HIF-1α signaling pathway. (c) Beclin-1 was measured by immunoblotting under normoxia and hypoxia. (d) Atg5 was measured by Western blotting and showed a similar trend to caspase-3. (e) HIF-1α was measured with Western blotting. The result revealed that HIF was involved in targeting apoptosis induced by autophagy.