Figure 4.

(A) CB monocytes were differentiated to macrophages (Mϕ) and seeded on 3wt%, 6wt%, and 14wt% hydrogels. (B) Macrophage cell proliferation was determined by enumerating the number cells present after 3 days of culture and normalizing to the number of cells initially seeded on day 0 (dashed line represents day 0) (C) Flow cytometric analysis of the percentage of macrophage staining positively for each phenotypic surface marker after 3 days of culture on hydrogels. (D) Quantification of secreted proteins from macrophage cultured on hydrogels for 3 days. In B-D, data are represented as the mean ± SEM, with n = 3 human donors per condition. A one-way ANOVA, followed by a Tukey HSD post hoc test, was used to detect statistical significance, *p<0.05, ns = no significance.