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. 2021 Feb 25;296:100470. doi: 10.1016/j.jbc.2021.100470

Figure 3.

Figure 3

Knockdown of Ugcg gene by DsiRNA reduced SARS-CoV-2 release to the medium.A, Ugcg silencing efficacy by DsiRNA in Vero E6 cells. Quantification of Ugcg mRNAs from nontargeted and Ugcg-specific DsiRNA (20 nM) transfected Vero E6 cells 24 h after transfection. mRNA levels were determined by real-time PCR. Mock transfected cells were used as control. Data are the means of triplicates ±SEM. ∗∗∗∗p < 0.0001. B, 24 h after DsiRNA transfection, cells were infected with SARS-CoV-2 at a multiplicity of infection (MOI) of 0.01. Twenty four hours after infection, viral release was measured through quantitation of SARS-CoV-2 RNA levels by real-time PCR. Data are the means of five replicates ±SEM. ∗∗p < 0.01. DsiRNA, Dicer-substrate siRNA; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2.