Skip to main content
. Author manuscript; available in PMC: 2021 Feb 25.
Published in final edited form as: J Am Chem Soc. 2020 Dec 23;143(1):176–183. doi: 10.1021/jacs.0c07879

Figure 5.

Figure 5.

Single turnover photochemical assays of the E52Q-photoβ2:α2 and E52Q/Y356F2Y-photoβ2:α2 complex with 10 μM α2, 10 μM photoβ2, 0.2 mM [3H]-CDP substrate, 3 mM ATP effector, and 10 mM Ru(NH3)6Cl3 in assay buffer with (red) and without (black) 10 min exposure to light (λ > 320 nm). Error bars represent one standard deviation among triplicate measurements.