Table 1.
The studies of disease modeling in iPSCs-derived organoids
| Organ | Disease | Induced cell types | Modeling | Reference |
|---|---|---|---|---|
| Lung | Infection with respiratory syncytial virus | Lung bud organoids (LBOs) | For RSV infection of day 170 LBOs, 107 plaque-forming units (PFU) of RSV in 1 l was directly added onto each organoid and incubated for 3 h at 37℃. | (14) |
| Fibrosis | LBOs | Mutation of HPS1 | (14) | |
| Infection with human para-influenza virus 3 (HPIV3) | LBOs | Recombinant HPIV3 for 32 days | (15) | |
| IPF | Mesenchymal organoids | 10 ng/ml TGF-β1 for 2 days | (42) | |
| Intestine | Salmonellae (bacterial) infection | intestinal organoids (iHO)-gut | Add of 1×106 bacteria/well in 24 well | (31) |
| Intestinal fibrosis | iHO -mid/hindgut | 2 ng/ml TGFβ for 96 hours | (32) | |
| Brain | Zika virus infection | Cerebral organoids | ZIKV for 7 days | (33) |
| Zika virus infection | Neural organoids | 3×105 PFU/ml 2 h at 37℃ | (34) | |
| Parkinson’s disease (PD) | Midbrain-like organoids | PD patient-derived organoids | (35) | |
| Alzheimer’s disease (AD) | Neural organoids | AD patient-derived organoids | (36) | |
| Liver | Steatohepatitis (fibrosis with inflammation) | Liver organoids | 800 mM oleic acid (OA) for 7 days | (37) |
| Infection with hepatitis B virus | Hepatic organoids | HBV (500, 5000 GEQ/cell) for 10 days | (39) |