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. 2020 Aug 20;12(1):101–113. doi: 10.1007/s13239-020-00483-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Total FITC-avidin accumulation, (b) percentage of permeable tricellular junctions, (c) percentage of the total tracer accumulation due to tricellular junctions, (d) mean tracer accumulation per permeable tricellular junction, and (e) confocal images (tracer accumulation, green; VE-cadherin, red), all for untreated HAEC monolayers or monolayers treated with S1P or thrombin. RFU relative fluorescence units. Bar = 100 μm.