Fig. 7.

Overexpresion of the PSBM4-interacting interfaces of Bsn in Bsn^GT cultures rescues the proteasome activity levels. a Chymotrypsin-, trypsin- and caspase-like activities are elevated in Bsn^GT cortical neurons as compared to the neurons from their WT littermates. Chymotrypsin- (b), trypsin- (c) and caspase-like (d) activities were significantly reduced upon expression of PSMB4-interacting fragments EGFP-Bsn2 or EGFP-Bsn4 in WT neurons. Importantly, there was no difference in proteasome activities between WT and Bsn^GT neurons expressing EGFP-Bsn2 or EGFP-Bsn4. Treatment with epoxomicin was always performed to validate the activity assay. In A, n number (in parentheses) denotes the number of experiments performed on independently prepared cultures. p-values versus control (WT-EGFP) were obtained by unpaired t test. Data are represented as boxes indicating the interquartile distance with median, whiskers minimum and maximum values, and mean showed as +. In b, c and d p values versus control (WT-EGFP) were obtained by one-way ANOVA with Tukey’s posttest