Extended Data Fig. 2. K48 and K63 chain-forming E2s equally discharge to ^K63UB_C1-C5 and K48UB_C1-C5 acceptors respectively.

(a) Di-UB formed by K48 UB chain-forming E2 UBE2G1 with ^K63UB_C1-C5 acceptors in the absence (left) or presence (right) of neddylated CRL4 (N8CRL4). (b) Di-UB formed by the K63 UB chain-forming E2 UBE2N/UBE2V1 complex with the ^K48UB_C1-C5 acceptors in the absence (left) or presence (right) of the E3 RNF4 RING domain. For all plots graphs, di-UB levels (μMol) represent the final time-points from the reactions (Source Data Extended Data Fig. 2), N=2 independent experiments. For samples derived from the same experiment, gels were processed in parallel.