Table 1.
Confidence levels (%) for MS identification of individual peptides derived from recombinant polypeptides of lrg-transfected MCF-7 cells*
| Tryptic Peptides | Supernate: 33 kD | Supernate: 50 kD | Cell Pellet: 45 kD |
|---|---|---|---|
| 1–6 (GalN 2) | - | - | - |
| 7–12 | 89 | 95 | 61 |
| 13–58 (GlcN 44) | - | - | - |
| 59–84 | 99 | 99 | 99 |
| 85–90 | 43 | - | 84 |
| 91–113 | - | - | - |
| 114–129 | - | - | 99 |
| 130–140 | 99 | 99 | 99 |
| 141–142 | - | - | - |
| 143–156 (GlcN 151) | 99 | - | - |
| 144–155 (GlcN 151) | 99 | - | - |
| 157–174 | 99 | 99 | 99 |
| 175–181 | 98 | 75 | 97 |
| 182–204 | 99 | 99 | - |
| 182–188 | - | - | - |
| 189–204 | 99 | - | - |
| 189–194 | - | - | - |
| 195–204 | 99 | 99 | 99 |
| 205–212 | 99 | 99 | 99 |
| 213–215 | - | - | - |
| 216–225 | 99 | 99 | 99 |
| 213–225 | - | - | - |
| 226–256 (GlcN 234) | - | - | - |
| 257–277 | 99 | - | 99 |
| 278–283 | - | - | - |
| 284–285 | - | - | - |
| 286–293 (GlcN 290) | - | - | - |
| 294–301 | - | - | - |
| 302–310 | - | - | - |
| 294–310 | 99 | - | - |
| 311–312 | - | - | - |
| Total Coverage | 74% | 55% | 57% |
*
Protein confidence levels as determined by Protein Pilot 2.0.1 software; peptide mass error range was 3–48 ppm