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. Author manuscript; available in PMC: 2021 Jul 4.
Published in final edited form as: Nat Chem Biol. 2021 Jan 4;17(3):291–297. doi: 10.1038/s41589-020-00702-5

Extended Data Fig. 6. Effect of intersubunit crosslinking on the CRD conformation.

Extended Data Fig. 6

Representative live-cell FRET measurement using HEK293T cells expressing 548UAA with the crosslinking mutation L521C upon application of intermediate and saturating glutamate. FRET signal is normalized using initial FRET at time = 0.