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. Author manuscript; available in PMC: 2021 Jul 4.
Published in final edited form as: Nat Chem Biol. 2021 Jan 4;17(3):291–297. doi: 10.1038/s41589-020-00702-5

Extended Data Fig. 9. Analysis of conformational state 2 and state 4.

Extended Data Fig. 9

Example single-molecule time traces of 548UAA YADA/WT heterodimers at 20 μM glutamate. ATTO488 (light blue), donor (green) and acceptor (red) intensities and the corresponding FRET (blue) are shown. Dashed lines represent 4 distinct FRET states. Data was acquired at 100 ms time resolution.