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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: Psychoneuroendocrinology. 2020 Dec 15;125:105110. doi: 10.1016/j.psyneuen.2020.105110

Figure 4.

Figure 4.

ANA-12 blocks estradiol-induced TrkB activation and accelerates increased mBDNF expression. TrkB and BDNF expression were assessed 4 hours after ICV infusion of vehicle or E2 (10 µg) and DH infusion of vehicle or ANA-12 (0.5 µg/hemisphere) in OVX female mice (A). Levels of TrkB mRNA were unaffected by E2 and ANA-12 treatment, but E2-induced TrkB phosphorylation was blocked by ANA-12 (B, n = 6–9/group). Levels of BDNF mRNA were significantly increased by E2 treatment and E2 and ANA-12 interacted to increase mBDNF protein at 4 hours post-infusion (C, n = 8–14/group). *p < 0.05 compared to vehicle control. Error bars indicate mean ± SEM.